6 Oct 2020 Knockdown of Ku70 or Ku80 in naïve mice elicited mitochondrial collapse or ER stress, leading to bronchial epithelial cell apoptosis and

First, either Ku70 or Ku80 functions outside the Ku heterodimer such that deletion of one is not identical to deletion of the other. Second, divergent genetic backgrounds or environments inﬂuence the phenotype. To distinguish between these possi-bilities, the Ku70 and Ku80 mutations were crossed together to generate Ku70, Ku80, and double

The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8). To test putative functions for Ku70, we have used gene-targeted mutation to generate a murine embryonic stem cell line which lacks Ku70 expression. We find that the Ku70 -/- cells produce no detectable Ku70 and very little Ku80, suggesting a direct interrelationship between their levels. Both Ku70 and Ku80 therefore contain monopartite NLSs, and sequences outside the basic cluster make favorable interactions with Impα, suggesting that this may be a general feature in monopartite NLSs. We show that the Ku70 NLS has a higher affinity for Impα than the Ku80 NLS, consistent with more extensive interactions in its N-terminal region. function of Ku70 at DSB sites might play a crucial role in modulating the NHEJ repair pathway [3, 14, 20, 26, 28]. There is, however, no report describing the localization and function of canine Ku70.

Ku70 and ku80 function

2011-10-01 · Ku, the heterodimer of Ku70 and Ku80, plays an essential role in the DNA double-strand break (DSB) repair pathway, i.e., non-homologous end-joining (NHEJ). Two isoforms of Ku80 encoded by the same genes, namely, Ku80 and KARP-1 are expressed and function in primate cells, but not in rodent cells. Ku80 works as a heterodimer with Ku70. Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK). Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA‐PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double‐strand breaks, and V(D)J recombination product formation defects.

2015-01-01

find that PAXX interacts with Ku threaded on DNA via the Ku70 subunit and requires a bare DNA extension for stability. They show that PAXX’s function overlaps with c-NHEJ factor XLF, explaining why its ablation has only a mild effect on NHEJ. Ku70 is the 70 kDa subunit of the lupus Ku autoantigen.

2003-06-15

We combine protein signatures 5 Mar 2021 Plays a role in the regulation of DNA virus-mediated innate immune response by Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. 1 Oct 2019 In the present study, to evaluate the role of Ku80 in thyroid carcinoma (TC), For example, the expression of Ku70/Ku80 has been shown to be 26 Mar 2002 These results suggest that the evolutionarily conserved Ku70–Ku80 heterodimer functions in DSB repair by the NHEJ pathway in A. thaliana.

LRIK interacts with the Ku70-Ku80 heterodimer enhancing the efficiency of NHEJ repair. Despite recent advances in our understanding of the function of long noncoding RNAs (lncRNAs), their roles and functions in DNA repair pathways remain poorly understood. By screening a panel of uncharacterized lncRNAs to identify those whose transcription is induced by double-strand breaks (DSBs), w …. In eukaryotes, Ku is a heterodimer comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end binding factor in the “classical” non-homologous end joining (C-NHEJ) pathway, the main DNA double-strand break (DSB) repair pathway in mammals.
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The EMBO Journal Vol.16 No.22 pp.6874–6885, 1997 Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions Shengfang Jin and David T.Weaver1 Division of The proteins Ku70 (69.8 kDa) and Ku80 (82.7 kDa) form a heterodimeric complex that is an essential component of the nonhomologous end joining DNA double-strand break repair pathway in mammalian cells. Interaction of Ku with DNA is central for the functions of Ku. 2001-03-01 · Single-stranded DNA-dependent ATP-dependent helicase.

Cellular retinaldehyde-binding/triple function domain-containing protein repair cross-complementing protein 5 OS=Dictyostelium discoideum GN=ku80 PE=3 ATP-dependent DNA helicase ku70 OS=Dictyostelium discoideum GN=ku70 Somatic mutations in nonhomologous end-joining (NHEJ) genes (DCLRE1C/ARTEMIS, PRKDC/DNA-PKcs, XRCC5/KU80, and XRCC6/KU70) were identified Ku heterodimer (Ku70 / Ku80) är den centrala DNA-bindande komponenten i that phosphorylation on Ku70 S155 in response to DNA damage functions to I NHEJ katalyseras ligering av DNA-ändarna efter DNA-proteinkinas komplex (DNA-PK katalytisk subenhet (DNA-PKcs), Ku70, Ku80) genom DNA-ligas IV i 86, 87 I NHEJ-vägen binder Ku70 och Ku80 DSB, följt av rekrytering och 156 Thus, in view of a fidelity control function of p53 in HR, it is conceivable that p53 Function.
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Purification of the Ku70/Ku80 dimer. Ku70 and Ku80 were purified from the HeLa nuclear extract (CilBiotech, Mons, Belgium) by using a combination of heparin, double‐stranded DNA cellulose, phenyl sepharose high resolution and monoQ chromatographies. DNA interaction assay.

The Ku70/80 heterodimer binds to DNA ends and attracts other proteins involved in the non-homologous end-joining (NHEJ) pathway of DNA double-strand break repair. We developed a novel assay to measure DNA binding and release kinetics using differences in Förster resonance energy transfer (FRET) of the ECFP-Ku70/EYFP-Ku80 heterodimer in soluble and DNA end bound states. Ku70 and Ku80 heterodimers function as regulatory subunits of the DNA-dependent protein kinase and play a very important role in the repairing of DNA double-strand breaks.

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Ku70 and Ku80 heterodimers function as regulatory subunits of the DNA-dependent protein kinase and play a very important role in the repairing of DNA double-strand breaks.

Ku70‐knockout mice, in contrast, appear to be tumor prone and develop thymic lymphomas with high incidence (30, 36). Finally, in agreement with a Ku70‐specific role in life‐span regulation, it was recently shown that knockdown of Ku70, but not Ku80, significantly extended life span in the p53‐null background . XRCC4, is a protein of unknown function. The Ku70 protein is an additional component of DNA-PK; Ku70 forms a het-erodimer with Ku80 to generate the DNA end-binding com-ponent of the enzyme. To test putative functions for Ku70, we have used gene-targeted mutation to generate a murine em-bryonic stem cell line which lacks Ku70 expression.

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Ku70 and Ku80 form the “Ku” DNA end-binding complex which functions as the DSB recognition com-ponent of C-NHEJ, whereas the XRCC4/ Lig4 complex is specific for C-NHEJ ligation. (2002) Korabiowska et al. Modern Pathology. Ku70 and Ku80 heterodimers function as regulatory subunits of the DNA-dependent protein kinase and play a very important role in the repairing of DNA double-strand breaks. Although Ku70 is proposed as a candidate for a … Structural Basis of Importin-α-Mediated Nuclear Transport for Ku70 and Ku80 Agnes A. S. Takeda1, Andrea C. de Barros1, Chiung-Wen Chang2, Boštjan Kobe2 and Marcos R. M. Fontes1⁎ 1Departamento de Física e Biofísica, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP 18618-970, Brazil 2School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience 2003-03-01 of the Ku70/80 heterodimer in vitro. We further deﬁned the function of the Ku70 and Ku80 C-terminal domains in DNA end binding and DNA-PKCS interaction.

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